M phase, also called as cell division, is the most crucial and fundamental affair of the eukaryotic cell cycle. After the chromosomes have been replicated during the S phase, the sister chromatids are separated and distributed into two daughter cells equally and faithfully. Also, each daughter cell receives the almost average and necessary intracellular constituents and organelles from the mother cell. Generally, cell division consists of six stages, including prophase, prometaphase, metaphase, anaphase, telophase and cytokinesis. And the first five stages constituent mitosis. During mitosis, numerous proteins organize protein super-complexes at the three distinct regions of centrosome, kinetochore/centromere and cleavage furrow/midbody. Although many proteins have been identified to be localized on centrosome, kinetochore and/or midbody, an integrated resource on this area still remains not to be available. In this work, we have collected all proteins identified to be localized on kinetochore, centrosome, and/or midbody from two fungi (S. cerevisiae and S. pombe) and five animals, including C. elegans, D. melanogaster, X. laevis, M. musculus and H. sapiens. From the related literature of PubMed, numerous proteins have been manually curated to be localized on at least one of the sub-cellular localizations of kinetochore, centrosome and midbody. And to promise the quality of data, based on the rationale of "Seeing is believing", these proteins have been unambiguously observed under fluorescent microscope as directly supportive evidences. Then an integrated and searchable database MiCroKit - Midbody, Centrosome and Kinetochore has been established. The MiCroKit database is the first integrative resource to pin point most of identified components and related scientific information of midbody, centrosome and kinetochore. The version 1.0 of MiCroKit database was set up on Nov. 2nd, 2005, containing 1,065 unique proteins. The MiCroKit version 2.0 was released on Jun. 5th, 2006, with 1,120 entries. Currently, the MiCroKit 3.0 database was updated on July 9, 2009, containing 1,489 unique protein entries. ... [Information of the supplier, modified]
The SEQanswers wiki engaging with sequencing and sequencing technology is a Semantic MediWiki (SMW) site that is edited and updated by the members of the SEQanswers community. The wiki provides an extensive catalogue of manually categorized analysis tools, technologies and information about service providers. Within two years, the SEQanswers community has created pages for over 400 unique software tools and associated them with around 350 literature references and 500 web links. SEQwiki contains data for 578 bioinformatics applications, 484 references, and 753 URLs. ... [Information of the supplier, modified]
YeTFaSCo stands for Yeast Transcription Factor Specificity Compendium. It is a collection of all available TF specificities for the yeast Saccharomyces cerevisiae in Position Frequency Matrix (PFM) or Position Weight Matrix (PWM) formats. With it, you can scan sequences with the motifs to find where potential binding sites lie, inspect precomputed genome-wide binding sites, find which TFs have a motif similar to one you have found, and download the collection of motifs. ... [Information of the supplier]
SEVENS summarizes GPCR (G-protein coupled receptor) genes that are identified with high accuracy from 56 eukaryote genomes, by a pipeline integrating such software as a gene finder, a sequence alignment tool, a motif and domain assignment tool, and a transmembrane helix predictor. This treats a larger data space (than that in currently available other databases), which should include not only the expressed sequences but also the newly identified sequences that cannot be detected by in vivo experiments, although they definitely exist on the genome sequence and are just waiting for the opportunity to express their functions. SEVENS can provides the infrastructure of general information of "GPCR universe" for comparative genomics. ... [Information of the supplier]
Bluejay is a set of Java(TM) objects for the processing, display and querying of XML encoded linear scale data. The main subject for analysis in our implementation are genetic and protein sequences, where features fall somewhere on the linear molecules. Bluejay is growing with the goal of creating a graphical genetic data viewer with point-and-click data mining capabilities, such as those available through your standard Web browser. If the image to the left was animated when you first loaded this document, you should have all you need. Otherwise click on the image to obtain the Java Runtime Environment. Please see the publications for more details on Bluejay. ... [Information of the supplier]
The Multi-purpose Automated Genome Project Investigation Environment (MAGPIE, http://magpie.ucalgary.ca/) is a software package for the automated curation and presentation of DNA and protein sequences. MAGPIE distills the results of multiple database searches for each sequence (e.g. expressed sequence tags, ESTs) or subsequence (e.g. open reading frames, ORFs, in bacterial genomic DNA) into a summary page that facilitates interpretation of genes' biological roles and origins. Database search tool used with MAGPIE include the standard NCBI-BLAST set of searches, plus more sensitive Smith-Waterman searches for frameshift prone data such as ESTs. Hidden Markov Model searches are also performed, including against an in-house HMM library derived from the NCBI's Clusters of Orthologuous Genes (COGs) database, including eukaryotic and prokaryotic clusters. HMM searches often provide single, concise, and accurate data for functional assignment. ... [Information of the supplier]
The 2013 IMB Conference will be the first major European meeting to focus on the latest findings and concepts in the fields of chromatin dynamics and stem cells. As such it will bring together experts in epigenetics, developmental biology and cellular reprogramming. The conference will be of interest both to researchers performing basic research in these fields and to applied scientists interested in cellular reprogramming and translational medicine. The conference is open to anyone interested. ... [Information of the supplier]
A comprehensive knowledge of all the factors involved in splicing, both proteins and RNAs, and of their interaction network is crucial for reaching a better understanding of this process and its functions. A large part of relevant information is buried in the literature or collected in various different databases. By hand-curated screenings of literature and databases we retrieved experimentally assessed data on 71 human RNA-binding splicing regulatory proteins and organized them into a database called “SpliceAid-F”. For each splicing factor (SF) the database reports its functional domains and its protein and chemical interactors. Furthermore, we collected experimentally validated RNA-SF interactions, including relevant information on the RNA binding sites such as the genes where these sites lie, their genomic coordinates, the splicing effects, experimental procedures, as well as the corresponding bibliographic references. We also collected information from experiments showing no RNA-SF binding, at least in the assayed conditions. In total, SpliceAid-F contains 4227 interactions, 2622 RNA binding sites and 1170 no-binding sites, including information on binding and no-binding specificity in different cellular contexts. Data collected in SpliceAid-F can provide significant information to explain an observed splicing pattern as well as the effect of mutations in functional regulatory elements. ... [Information of the supplier]
The Encyclopedia of DNA Elements (ENCODE) consortium aims to identify all functional elements in the human genome sequence. These elements include genomic regions bound by transcription factors (TFs), occupied by nucleosomes, occupied by nucleosomes with modified histones, hypersensitive to cleavage of DNase I, etc. We organize the on-going analysis results of TF binding data in the web accessible repository factorbook.org. ... [Information of the supplier]
ChIPBase, an integrated resource and platform for decoding transcription factor binding maps, expression profiles and transcriptional regulation of long non-coding RNAs (lncRNAs, lincRNAs), microRNAs, other ncRNAs(snoRNAs, tRNAs, snRNAs, etc.) and protein-coding genes from ChIP-Seq data. ChIPBase currently includes millions of transcription factor binding sites (TFBSs) among 6 species. ChIPBase provides several web-based tools and browsers to explore TF-lncRNA, TF-miRNA, TF-mRNA, TF-ncRNA and TF-miRNA-mRNA regulatory networks. ... [Information of the supplier]
