The membrane organization and subcellular location of a protein can provide information about its functional role. Historically, these data have been difficult to produce on a large scale for higher eukaryotic organisms. However, recent advances in membrane organization prediction methods and high-throughput subcellular localization assays have made it possible to generate these datasets. LOCATE is a curated, web-accessible database that houses data describing the membrane organization and subcellular localization of proteins from the FANTOM3 Isoform Protein Sequence set. Membrane organization is predicted by the high-throughput, computational pipeline MemO. The subcellular locations of selected proteins from this set were determined by a high-throughput, immunofluorescence-based assay and by manually reviewing over 1700 peer-reviewed publications. The results of the MemO pipeline and the subcellular localization methods are stored in the database with supporting information including a graphical depiction of the membrane organization juxtaposed with InterPro-predicted features and links to several external databases. The database is searchable by subcellular location, protein class, descriptive keyword, and sequence similarity. The data are retrievable in human- and machine-readable formats and in batch. ... [Information of the supplier]
TCDB is a curated database of factual information from over 10,000 published references. The database contains about 3,000 protein sequences. The database details a comprehensive IUBMB approved classification system for membrane transport proteins known as the Transporter Classification (TC) system. The TC system is analogous to the Enzyme Commission (EC) system for classification of enzymes, but incorporates phylogenetic information additionally. TCDB is operated by the Saier Lab Bioinformatics Group. ... [Information of the supplier]
The European Molecular Biology Laboratory is a non-profit organisation and a basic research institute funded by public research monies from 18 member states. Research at EMBL is conducted by approximately 80 independent groups covering the spectrum of molecular biology. The cornerstones of EMBL's mission are: to perform basic research in molecular biology, to train scientists, students and visitors at all levels, to offer vital services to scientists in the member states, and to develop new instruments and methods in the life sciences, and technology transfer. ... [Information of the supplier]
The BioCurrents Research Center (BRC) specializes in the development and utilization of a range of technologies to examine the chemical profiles of the living cell, specifically within the complex and dynamic boundary layer. The technologies are employed by in-house researchers and collaborators from around the world to address a range of human health issues including diabetes, infertility and degenerative diseases. ... [Information of the supplier]
Mitose, Funktionen von Zellorganellen und andere auf Zellebene lokalisierte Vorgänge sind das Thema kurzer Videosequenzen und Animationen, die über diese Website erreichbar sind. [Redaktion vifabio]
CELLS alive! represents 30 years of capturing film and computer-enhanced images of living cells and organisms for education and medical research. The site has been available continuously and updated annually since May of 1994 and now hosts over 4 million visitors a year. All text, images, and layout are provided by me, Jim Sullivan. The majority of the site is free of cost and registration for anyone with internet access. With up to 25,000 visitors a day during the school year, hosting costs are an issue. Students and teachers who purchase CDROMs and downloads for classroom use make this possible. A stock video library is also available, providing producers with a range of subjects, including both live recording and computer animation. Immune cells, bacteria, parasites, and aquatic organisms are available for licensing for educational, broadcast, and commercial use. ... [Information of the supplier]
We present a neural network based method (ChloroP) for identifying chloroplast transit peptides and their cleavage sites. Using cross-validation, 88% of the sequences in our homology reduced training set were correctly classified as transit peptides or nontransit peptides. This performance level is well above that of the so far only publicly available chloroplast localization predictor PSORT. Cleavage sites are predicted using a scoring matrix derived by an automatic motif-finding algorithm. Approximately 60% of the known cleavage sites in our sequence collection were predicted to within +- 2 residues from the cleavage sites given in SWISS-PROT. An analysis of 715 A. thaliana sequences from SWISS-PROT suggests that the ChloroP method should be useful for the identification of putative transit peptides in genome-wide sequence data. ... [Information of the supplier]
GOBASE is a taxonomically broad organelle genome database that organizes and integrates diverse data related to mitochondria and chloroplasts. In its next phase, GOBASE will also include information on representative bacteria that are thought to be specifically related to the bacterial ancestors of mitochondria and chloroplasts ... [Information of the supplier]
MPtopo is a curated database of membrane proteins with experimentally validated transmembrane (TM) segments. The database is maintained in SQL form using the MySQL server. It can be searched using MPtopoQuerier, a java applet developed for this purpose. Retrieved sequences can be examined automatically by MPEx, a java-based hydropathy-plot tool. MPtopo currently contains a total of 167 proteins with 1028 TM segments. ... [Information of the supplier, modified]
Die Site der Max-Planck-Gesellschaft stellt mit kurzen Artikeln, Videos und Graphiken Themen aus dem Bereich der biochemischen Genetik, Zellbiologie und Medizin vor. Ein umfangreiches Glossar der Zellbiologie und einzelne Spiele gehören zum Angebot. Parallel zur Website wurde 2001/2002 die Ausstellung „Virtuelle Zelle“ gezeigt. [Redaktion vifabio]
